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Addgene

PB-CRX-T2A-NeuroD1
(Plasmid #194607)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194607 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-TA-ERN (Addgene #80474 )
  • Backbone size w/o insert (bp) 9614
  • Total vector size (bp) 11636
  • Vector type
    Mammalian Expression ; piggyBac transposon
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRX-T2A-NeuroD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2022
  • GenBank ID
    NM_000554(CRX) NM_002500.5 (NeuroD1)
  • Promoter tetO

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-CRX-T2A-NeuroD1 was a gift from Haruhisa Inoue (Addgene plasmid # 194607 ; http://n2t.net/addgene:194607 ; RRID:Addgene_194607)

  • For your References section:

    One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors. Otsuka Y, Imamura K, Oishi A, Kondo T, Suga M, Yada Y, Shibukawa R, Okanishi Y, Sagara Y, Tsukita K, Tsujikawa A, Inoue H. iScience. 2022 Mar 8;25(4):103987. doi: 10.1016/j.isci.2022.103987. eCollection 2022 Apr 15. 10.1016/j.isci.2022.103987 PubMed 35330684
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