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pBABE Gal4(1-94) HSF ABC Flag ac
(Plasmid #1946)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Heat Shock Factor 1
  • Alt name
    HSF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    acidic mutant (D416, E493, E496 A)
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Tags / Fusion Proteins
    • flag (N terminal on insert)
    • Gal4 DBD (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Carl Wu

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains the FLAG epitope amino terminal to the GAL4 DNA binding domain (amino acids 1 to 94), attached to the C terminus of hHSF1, (amino acids 201 to 529). This construct was subcloned into the pBABE/puro retroviral vector via the BglII and EcoRI sites. Acidic Mutant. (Kingston #1153)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE Gal4(1-94) HSF ABC Flag ac was a gift from Robert Kingston (Addgene plasmid # 1946 ; http://n2t.net/addgene:1946 ; RRID:Addgene_1946)

  • For your References section:

    Transcriptional activation domains of human heat shock factor 1 recruit human SWI/SNF. Sullivan EK, Weirich CS, Guyon JR, Sif S, Kingston RE. Mol Cell Biol 2001 Sep;21(17):5826-37. 10.1128/MCB.21.17.5826-5837.2001 PubMed 11486022
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