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Addgene

pcDNA216-CoV2-RBD-beta variant
(Plasmid #194598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA216
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoV2-RBD-beta variant
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA216-CoV2-RBD-beta variant was a gift from Ben Murrell (Addgene plasmid # 194598 ; http://n2t.net/addgene:194598 ; RRID:Addgene_194598)

  • For your References section:

    Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models. Sheward DJ, Mandolesi M, Urgard E, Kim C, Hanke L, Perez Vidakovics L, Pankow A, Smith NL, Castro Dopico X, McInerney GM, Coquet JM, Karlsson Hedestam GB, Murrell B. Cell Rep Med. 2021 Nov 16;2(11):100450. doi: 10.1016/j.xcrm.2021.100450. Epub 2021 Oct 23. 10.1016/j.xcrm.2021.100450 PubMed 34723224
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