hIgG1-Ty1-hFc
(Plasmid
#194593)
-
PurposeMammalian expression of Ty1 nanobody upstream of human IgG1
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 194593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonehIgG1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTy1-hFc
-
SpeciesOther
-
Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
hIgG1-Ty1-hFc was a gift from Gerald McInerney (Addgene plasmid # 194593 ; http://n2t.net/addgene:194593 ; RRID:Addgene_194593) -
For your References section:
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction. Hanke L, Vidakovics Perez L, Sheward DJ, Das H, Schulte T, Moliner-Morro A, Corcoran M, Achour A, Karlsson Hedestam GB, Hallberg BM, Murrell B, McInerney GM. Nat Commun. 2020 Sep 4;11(1):4420. doi: 10.1038/s41467-020-18174-5. 10.1038/s41467-020-18174-5 PubMed 32887876