pHEN-CoV2-nanobody-Ty1
(Plasmid
#194588)
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PurposeBacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHEN
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHEN-CoV2-nanobody-Ty1 was a gift from Gerald McInerney (Addgene plasmid # 194588 ; http://n2t.net/addgene:194588 ; RRID:Addgene_194588) -
For your References section:
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction. Hanke L, Vidakovics Perez L, Sheward DJ, Das H, Schulte T, Moliner-Morro A, Corcoran M, Achour A, Karlsson Hedestam GB, Hallberg BM, Murrell B, McInerney GM. Nat Commun. 2020 Sep 4;11(1):4420. doi: 10.1038/s41467-020-18174-5. 10.1038/s41467-020-18174-5 PubMed 32887876