pRSII41N-TDH3pr-mNeon
(Plasmid
#194536)
-
PurposeLow copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 194536 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRSII41N-TDH3pr
-
Vector typeYeast Expression
-
Selectable markersNourseothricin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namenatAC
-
SpeciesS. cerevisiae (budding yeast)
- Promoter TDH3
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRSII41N-TDH3pr-mNeon was a gift from Patrick Gibney (Addgene plasmid # 194536 ; http://n2t.net/addgene:194536 ; RRID:Addgene_194536) -
For your References section:
Auxotrophy-Independent Plasmid Shuttle Vectors for Applications in Diverse Yeasts. Smith JR, Sislak CD, Fernandez Mendoza P, Carmichael L, Lewis AG, Chen A, Jiang GZ, Gibney PA. Appl Microbiol. 2024; 4(1):453-469. doi: 10.3390/applmicrobiol4010031 10.3390/applmicrobiol4010031