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Addgene

Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2
(Plasmid #194515)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194515 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    E1b-GFP-Tol2
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    EGFP
  • Species
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Eye lens-specific cryaa:mCherry cassette serves as transgenesis marker

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2 was a gift from Junsu Kang (Addgene plasmid # 194515 ; http://n2t.net/addgene:194515 ; RRID:Addgene_194515)

  • For your References section:

    Regeneration and developmental enhancers are differentially compatible with minimal promoters. Begeman IJ, Emery B, Kurth A, Kang J. Dev Biol. 2022 Sep 24;492:47-58. doi: 10.1016/j.ydbio.2022.09.007. 10.1016/j.ydbio.2022.09.007 PubMed 36167150
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