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Addgene

pGTag NGFR EGFP nLAP -1
(Plasmid #194506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS-
  • Total vector size (bp) 5227
  • Vector type
    Bacterial Cloning Vector
  • Selectable markers
    NGFR for affinity purification

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NGFR-P2A-nLAP(EGFP)
  • Insert Size (bp)
    1890

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTag NGFR EGFP nLAP -1 was a gift from Frank Schnütgen (Addgene plasmid # 194506 ; http://n2t.net/addgene:194506 ; RRID:Addgene_194506)

  • For your References section:

    Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR. Seibert M, Kurrle N, Stolp V, Nurnberger H, Tzschentke S, Borner L, Wempe F, Serve H, Schnutgen F. MethodsX. 2022 Nov 18;9:101929. doi: 10.1016/j.mex.2022.101929. eCollection 2022. 10.1016/j.mex.2022.101929 PubMed 36444289
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