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Addgene

pHAGE-EFS-foldon-GFP- PCP
(Plasmid #194503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Backbone size w/o insert (bp) 5882
  • Total vector size (bp) 7453
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    foldon-GFP-PCP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1305
  • Promoter EF-1α core promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taaggtgaacgcgtgcaggctggcgccaccatgggctacatccccgaggcccccagggac
  • 3′ sequencing primer ctcgaggctcccatcgatgccagagcggccgccagaacttccggatccacctccactagt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EFS-foldon-GFP- PCP was a gift from Chunqing Song (Addgene plasmid # 194503 ; http://n2t.net/addgene:194503 ; RRID:Addgene_194503)

  • For your References section:

    CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification. Lyu XY, Deng Y, Huang XY, Li ZZ, Fang GQ, Yang D, Wang FL, Kang W, Shen EZ, Song CQ. Cell Res. 2022 Nov;32(11):969-981. doi: 10.1038/s41422-022-00712-z. Epub 2022 Sep 14. 10.1038/s41422-022-00712-z PubMed 36104507
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