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Addgene

pAS162
(Plasmid #194485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYKT095
  • Backbone size w/o insert (bp) 1870
  • Total vector size (bp) 5285
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synGR Cassette
  • Species
    Synthetic
  • Insert Size (bp)
    3415

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GAGCCTATGGAAAAACGCCA
  • 3′ sequencing primer GATTATCAAAAAGGATCTTCACCTAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS162 was a gift from Ahmad Khalil (Addgene plasmid # 194485 ; http://n2t.net/addgene:194485 ; RRID:Addgene_194485)

  • For your References section:

    A Toolkit for Precise, Multigene Control in Saccharomyces cerevisiae. Sanford A, Kiriakov S, Khalil AS. ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423. 10.1021/acssynbio.2c00423 PubMed 36367334
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