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Addgene

pSDM177
(Plasmid #194476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHERD30T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    crRNA(Lse)
  • gRNA/shRNA sequence
    ctcggtacccggggatcctc

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSDM177 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 194476 ; http://n2t.net/addgene:194476 ; RRID:Addgene_194476)

  • For your References section:

    Bacteriophage genome engineering with CRISPR-Cas13a. Guan J, Oromi-Bosch A, Mendoza SD, Karambelkar S, Berry JD, Bondy-Denomy J. Nat Microbiol. 2022 Oct 31. pii: 10.1038/s41564-022-01243-4. doi: 10.1038/s41564-022-01243-4. 10.1038/s41564-022-01243-4 PubMed 36316452
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