pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G
(Plasmid
#194463)
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PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRRLSIN.cPPT
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Vector typeLentiviral
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Selectable markersZeocin ; GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAnti-JAG1 CAR
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G was a gift from Ana Sofia Coroadinha (Addgene plasmid # 194463 ; http://n2t.net/addgene:194463 ; RRID:Addgene_194463) -
For your References section:
Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors. Silva G, Rodrigues AF, Ferreira S, Matos C, Eleuterio RP, Marques G, Kucheryava K, Lemos AR, Sousa PMF, Castro R, Barbas A, Simao D, Alves PM. Biomolecules. 2023 Mar 2;13(3):459. doi: 10.3390/biom13030459. 10.3390/biom13030459 PubMed 36979394
