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Addgene

pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
(Plasmid #194457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLSIN.cPPT
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Anti-CD19 CAR
  • Species
    H. sapiens (human), M. musculus (mouse)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G was a gift from Ana Sofia Coroadinha (Addgene plasmid # 194457 ; http://n2t.net/addgene:194457 ; RRID:Addgene_194457)

  • For your References section:

    Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors. Silva G, Rodrigues AF, Ferreira S, Matos C, Eleuterio RP, Marques G, Kucheryava K, Lemos AR, Sousa PMF, Castro R, Barbas A, Simao D, Alves PM. Biomolecules. 2023 Mar 2;13(3):459. doi: 10.3390/biom13030459. 10.3390/biom13030459 PubMed 36979394
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