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Addgene

pUC57 β-Globin
(Plasmid #194437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    In situ Hybridization

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β-globin
  • Species
    D. rerio (zebrafish)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer ACC CTC ACT AAA GGG AAC GCG CAG CGA TTC AGA ACA T
  • 3′ sequencing primer ACG ACT CAC TAT AGG GCC TTC TGA GGG CTG ACA CAA CA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57 β-Globin was a gift from Christopher Sansam (Addgene plasmid # 194437 ; http://n2t.net/addgene:194437 ; RRID:Addgene_194437)

  • For your References section:

    Zebrafish Rif1 impacts zygotic genome activation, replication timing, and sex determination. Masser EA, Noble TD, Siefert JC, Goins D, Sansam CG, Sansam CL. eLife 2023 (Reviewed Preprint) 12:RP87671 10.7554/eLife.87671.1
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