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pLEXSY-hyg2.1_LtIFT43_Flag
(Plasmid #194433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEXSY-hyg2.1
  • Backbone manufacturer
    JenaBioscience
  • Backbone size w/o insert (bp) 8719
  • Total vector size (bp) 9829
  • Modifications to backbone
    Added a single XbaI site, linker sequence and 3X flag tag to the 3' of MCS
  • Vector type
    Leishmania tarentolae expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Intraflagellar transport complex subunit 43
  • Alt name
    IFT43
  • Species
    L. tarentolae
  • Insert Size (bp)
    1110
  • Promoter L.donovani promoter elements
  • Tags / Fusion Proteins
    • 3X Flag (C terminal on backbone)
    • Linker (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BgIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CCTTGCCACCAGATCTGCCATGTCCACGACTTCTATGACTGCC
  • 3′ sequencing primer CCGGAGCCTCTAGACTTCGAAACCTGCTTGGATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEXSY-hyg2.1_LtIFT43_Flag was a gift from Alan Brown (Addgene plasmid # 194433 ; http://n2t.net/addgene:194433 ; RRID:Addgene_194433)

  • For your References section:

    Mechanism of IFT-A polymerization into trains for ciliary transport. Meleppattu S, Zhou H, Dai J, Gui M, Brown A. Cell. 2022 Dec 22;185(26):4986-4998.e12. doi: 10.1016/j.cell.2022.11.033. 10.1016/j.cell.2022.11.033 PubMed 36563665
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