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Addgene

LT2_32_PyruEth_X2site
(Plasmid #194428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MoClo YTK
  • Vector type
    Yeast Expression ; Used as donor DNA in genome integration after linearisation with NotI

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PyruEth
  • Alt name
    pPDC1-ymYPET-tCYC1 + pADH2-mTurquoise2-tCYC1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sequence for mTurquoise2 obtained from Addgene plasmid #65139; Sequence for ymYPET obtained from Addgene plasmid #118455; Sequence for pTEFmut8 amplified from Addgene plasmid #124582.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LT2_32_PyruEth_X2site was a gift from Lisbeth Olsson (Addgene plasmid # 194428 ; http://n2t.net/addgene:194428 ; RRID:Addgene_194428)

  • For your References section:

    ScEnSor Kit for Saccharomyces cerevisiae Engineering and Biosensor-Driven Investigation of the Intracellular Environment. Torello Pianale L, Olsson L. ACS Synth Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124. 10.1021/acssynbio.3c00124 PubMed 37552581
Included in kit:
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