LT1_75_Donor_Tag_RPL13A-mTurq2
(Plasmid
#194415)
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PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMoClo YTK
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Vector typeYeast Expression, Synthetic Biology ; Used as donor DNA in genome integration after linearisation with NotI
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTurquoise2 CDS
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SpeciesS. cerevisiae (budding yeast), Synthetic
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MutationNA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySequence for mTurquoise2 obtained from Addgene plasmid #65139
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LT1_75_Donor_Tag_RPL13A-mTurq2 was a gift from Lisbeth Olsson (Addgene plasmid # 194415 ; http://n2t.net/addgene:194415 ; RRID:Addgene_194415) -
For your References section:
ScEnSor Kit for Saccharomyces cerevisiae Engineering and Biosensor-Driven Investigation of the Intracellular Environment. Torello Pianale L, Olsson L. ACS Synth Biol. 2023 Aug 8. doi: 10.1021/acssynbio.3c00124. 10.1021/acssynbio.3c00124 PubMed 37552581