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Addgene

pCMV-ADARcd-YTHD422N
(Plasmid #194402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Total vector size (bp) 5158
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADARcd fused to YTHD422N
  • Species
    H. sapiens (human)
  • Mutation
    D422N mutation in the YTH domain to enhance m6A binding
  • Entrez Gene
    ADAR (a.k.a. ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136)
  • Tag / Fusion Protein
    • ADARcd-YTHD422N-HA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-ADARcd-YTHD422N was a gift from Kate Meyer (Addgene plasmid # 194402 ; http://n2t.net/addgene:194402 ; RRID:Addgene_194402)

  • For your References section:

    Improved Methods for Deamination-Based m(6)A Detection. Zhu H, Yin X, Holley CL, Meyer KD. Front Cell Dev Biol. 2022 Apr 27;10:888279. doi: 10.3389/fcell.2022.888279. eCollection 2022. 10.3389/fcell.2022.888279 PubMed 35573664
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