pMIAII-Cd4
(Plasmid
#194401)
-
PurposeRetroviral expression of mouse CD4
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 194401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMIAII
-
Backbone manufacturerAddgene #52113
- Backbone size w/o insert (bp) 6476
-
Vector typeRetroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCd4
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1374
-
MutationWT
-
Entrez GeneCd4 (a.k.a. L3T4, Ly-4)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pLXSN
- 3′ sequencing primer IRES_rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMIAII-Cd4 was a gift from Michael Carroll (Addgene plasmid # 194401 ; http://n2t.net/addgene:194401 ; RRID:Addgene_194401) -
For your References section:
T cell help shapes B cell tolerance. Akama-Garren EH, Yin X, Prestwood TR, Ma M, Utz PJ, Carroll MC. Sci Immunol. 2024 Feb 16;9(92):eadj7029. doi: 10.1126/sciimmunol.adj7029. Epub 2024 Feb 16. 10.1126/sciimmunol.adj7029 PubMed 38363829