Skip to main content
Addgene

GST-14-3-3wt (pGEX)
(Plasmid #1944)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1944 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-KG
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 zeta
  • Alt name
    14.3.3
  • Alt name
    Ywhaz
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ywhaz (a.k.a. 1110013I11Rik, 14-3-3zeta)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-14-3-3wt (pGEX) was a gift from Joseph Avruch (Addgene plasmid # 1944)

  • For your References section:

    Identification of the 14.3.3 zeta domains important for self-association and Raf binding. Luo ZJ, Zhang XF, Rapp U, Avruch J. J Biol Chem 1995 Oct 6;270(40):23681-7. 10.1074/jbc.270.40.23681 PubMed 7559537
Related items: