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Addgene

NFfshb-t2a-GFP
(Plasmid #194356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 8119
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    follicle stimulating hormone beta subunit
  • Alt name
    fshb
  • Species
    Nothobranchius furzeri
  • Insert Size (bp)
    351
  • Tag / Fusion Protein
    • t2a-GFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGCAACTGGTTGTCATGGCAGC
  • 3′ sequencing primer ACAGCCGAGTACGTGTGGATGGAAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NFfshb-t2a-GFP was a gift from Itamar Harel (Addgene plasmid # 194356 ; http://n2t.net/addgene:194356 ; RRID:Addgene_194356)

  • For your References section:

    A scalable and tunable platform for functional interrogation of peptide hormones in fish. Moses E, Franek R, Harel I. eLife. 2023 Oct 24;12:e85960. doi: 10.7554/eLife.85960. 10.7554/eLife.85960 PubMed 37872843
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