pAAV-CMV-H2B-3xFLAG-2A-G(N2c)
(Plasmid
#194355)
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PurposeAAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameH2B-3xFLAG
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Tag
/ Fusion Protein
- FLAG
Gene/Insert 2
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Gene/Insert nameRabies CVS-N2c glycoprotein (G)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-H2B-3xFLAG-2A-G(N2c) was a gift from Marco Tripodi (Addgene plasmid # 194355 ; http://n2t.net/addgene:194355 ; RRID:Addgene_194355) -
For your References section:
Combining long-term circuit mapping and network transcriptomics with SiR-N2c. Lee H, Ciabatti E, Gonzalez-Rueda A, Williams E, Nugent F, Mookerjee S, Morgese F, Tripodi M. Nat Methods. 2023 Apr;20(4):580-589. doi: 10.1038/s41592-023-01787-1. Epub 2023 Mar 2. 10.1038/s41592-023-01787-1 PubMed 36864202