pSDM052
(Plasmid
#194341)
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PurposepUC mini-Tn7 vector expressing LseCas13a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18-mini-Tn7T-LAC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas13a(Lse)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSDM052 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 194341 ; http://n2t.net/addgene:194341 ; RRID:Addgene_194341) -
For your References section:
A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases. Mendoza SD, Nieweglowska ES, Govindarajan S, Leon LM, Berry JD, Tiwari A, Chaikeeratisak V, Pogliano J, Agard DA, Bondy-Denomy J. Nature. 2020 Jan;577(7789):244-248. doi: 10.1038/s41586-019-1786-y. Epub 2019 Dec 9. 10.1038/s41586-019-1786-y PubMed 31819262