pCS2-Lifeact-mRuby
(Plasmid #194339)

• Purpose: Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos
• Depositing Lab: Heidi Hehnly
• Publication: Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765. doi: 10.1371/journal.pgen.1010765. eCollection 2023 May.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4067
• Total vector size (bp): 4849
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lifeact
• Insert Size (bp): 51
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • mRuby (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: sp6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lifeact-mRuby 2 was purchased from Addgene, plasmid # 54674.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry