pCS2-FLAG-Rab8
(Plasmid
#194335)
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PurposeExpresses FLAG tagged Rab8 in mammalian cells and zebrafish embryos
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4067
- Total vector size (bp) 4777
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab8
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SpeciesCanis lupus
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Insert Size (bp)621
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Entrez GeneRAB8A (a.k.a. RAB8)
- Promoter CMV IE94
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-FLAG-Rab8 was a gift from Heidi Hehnly (Addgene plasmid # 194335 ; http://n2t.net/addgene:194335 ; RRID:Addgene_194335) -
For your References section:
Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. Aljiboury AA, Ingram E, Krishnan N, Ononiwu F, Pal D, Manikas J, Taveras C, Hall NA, Da Silva J, Freshour J, Hehnly H. PLoS Genet. 2023 May 15;19(5):e1010765. doi: 10.1371/journal.pgen.1010765. eCollection 2023 May. 10.1371/journal.pgen.1010765 PubMed 37186603