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Addgene

pCS2-CIB1-mCherry-Rab8A
(Plasmid #194333)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194333 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4067
  • Total vector size (bp) 5960
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CIB1
  • Alt name
    cryptochrome-interacting basic-helix-loop-helix 1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    516
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV IE94

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Rab8a
  • Species
    Canis Lupus
  • Insert Size (bp)
    621
  • Entrez Gene
    RAB8A (a.k.a. RAB8)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • mcherry (N terminal on insert)

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-CIB1-mCherry-Rab8A was a gift from Heidi Hehnly (Addgene plasmid # 194333 ; http://n2t.net/addgene:194333 ; RRID:Addgene_194333)
  • For your References section:

    Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. Aljiboury AA, Ingram E, Krishnan N, Ononiwu F, Pal D, Manikas J, Taveras C, Hall NA, Da Silva J, Freshour J, Hehnly H. PLoS Genet. 2023 May 15;19(5):e1010765. doi: 10.1371/journal.pgen.1010765. eCollection 2023 May. 10.1371/journal.pgen.1010765 PubMed 37186603