pSBtet rtTA G72V SE Δsplice GP Luc
(Plasmid #194328)

• Purpose: Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
• Depositing Lab: Alexander McLellan
• Publication: Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii: genes13101679. doi: 10.3390/genes13101679.

Backbone

• Vector backbone: pSBtet
• Backbone size w/o insert (bp): 6368
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418) ; EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Luciferase
• Species: Synthetic
• Insert Size (bp): 1653
• Mutation: Tet-responsive promotor related mutations: G72V in rtTA, Δsplice sites, sensitivity enhancing mutations
• Promoter: Tight tet-responsive promotor

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pSBtet rtTA G72V SE Δsplice GP Luc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry