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Addgene

AAVS1-Bxb1-LP-v2-TC
(Plasmid #194326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322/pUC
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 8200
  • Vector type
    Mammalian Expression, Cre/Lox, Synthetic Biology
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-EBFP2-attP-BleoR-lox251
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-Bxb1-LP-v2-TC was a gift from Richard Davis (Addgene plasmid # 194326 ; http://n2t.net/addgene:194326 ; RRID:Addgene_194326)

  • For your References section:

    Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads. Blanch-Asensio A, van der Vaart B, Vinagre M, Groen E, Arendzen C, Freund C, Geijsen N, Mummery CL, Davis RP. Stem Cell Res. 2023 Feb;66:102991. doi: 10.1016/j.scr.2022.102991. Epub 2022 Dec 5. 10.1016/j.scr.2022.102991 PubMed 36495703
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