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Addgene

pGTag Hygro BFP nLAP +2
(Plasmid #194319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS-
  • Total vector size (bp) 5391
  • Vector type
    Bacterial cloning vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hygro-P2A-nLAP(BFP)
  • Insert Size (bp)
    2052

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTag Hygro BFP nLAP +2 was a gift from Frank Schnütgen (Addgene plasmid # 194319 ; http://n2t.net/addgene:194319 ; RRID:Addgene_194319)

  • For your References section:

    CRISPR/Cas9-mediated generic protein tagging in mammalian cells. Thone FMB, Kurrle NS, von Melchner H, Schnutgen F. Methods. 2019 Jul 15;164-165:59-66. doi: 10.1016/j.ymeth.2019.02.018. Epub 2019 Feb 22. 10.1016/j.ymeth.2019.02.018 PubMed 30797903
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