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Addgene

pET-MhCINS-6xHis
(Plasmid #194304)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194304 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5150
  • Total vector size (bp) 6758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MhCINS
  • Alt name
    MhTPS
  • Species
    Meiogyne heteropetala
  • Insert Size (bp)
    1608
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TCTCTATCTAGAAGGAGGTATAATATGCGGAGATCTGCTAATTATC
  • 3′ sequencing primer GTGATGGGATCCGCCGTCAAGTGGTATTGGTTCAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-MhCINS-6xHis was a gift from Richard Saunders (Addgene plasmid # 194304 ; http://n2t.net/addgene:194304 ; RRID:Addgene_194304)

  • For your References section:

    Aerial litter mimicry: A novel form of floral deception mediated by a monoterpene synthase. Liu M-F, Chen J, Goodrich KR, Chiu SK, Pang CC, Scharaschkin T, Saunders RMK. J Ecology 2024; 00, 1–20. doi: 10.1111/1365-2745.14446 10.1111/1365-2745.14446
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