pCDNA3_LuxSit_i_mTagBFP2
(Plasmid
#194300)
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PurposeMammalian expression of the de novo luciferase, LuxSit-i
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 5900
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuxSit-i
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SpeciesSynthetic
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Insert Size (bp)357
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Tag
/ Fusion Protein
- mTagBFP2 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3_LuxSit_i_mTagBFP2 was a gift from David Baker & Andy Yeh (Addgene plasmid # 194300 ; http://n2t.net/addgene:194300 ; RRID:Addgene_194300) -
For your References section:
De novo design of luciferases using deep learning. Yeh AH, Norn C, Kipnis Y, Tischer D, Pellock SJ, Evans D, Ma P, Lee GR, Zhang JZ, Anishchenko I, Coventry B, Cao L, Dauparas J, Halabiya S, DeWitt M, Carter L, Houk KN, Baker D. Nature. 2023 Feb;614(7949):774-780. doi: 10.1038/s41586-023-05696-3. Epub 2023 Feb 22. 10.1038/s41586-023-05696-3 PubMed 36813896
