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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEBG
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name14-3-3 zeta
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Alt name14.3.3
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Alt nameYwhaz
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SpeciesM. musculus (mouse)
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MutationDimerization-deficient mutant of 14-3-3zeta aa: E5K, L12AE to Q12QR, Y82Q, K85N, and E87Q
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Entrez GeneYwhaz (a.k.a. 1110013I11Rik, 14-3-3zeta)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-14-3-3dm (pEBG) was a gift from Joseph Avruch (Addgene plasmid # 1943 ; http://n2t.net/addgene:1943 ; RRID:Addgene_1943) -
For your References section:
A dimeric 14-3-3 protein is an essential cofactor for Raf kinase activity. Tzivion G, Luo Z, Avruch J. Nature 1998 Jul 2;394(6688):88-92. 10.1038/27938 PubMed 9665134