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pET29_LuxSit_i
(Plasmid #194299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5800
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LuxSit-i
  • Species
    Synthetic
  • Insert Size (bp)
    417

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29_LuxSit_i was a gift from David Baker & Andy Yeh (Addgene plasmid # 194299 ; http://n2t.net/addgene:194299 ; RRID:Addgene_194299)

  • For your References section:

    De novo design of luciferases using deep learning. Yeh AH, Norn C, Kipnis Y, Tischer D, Pellock SJ, Evans D, Ma P, Lee GR, Zhang JZ, Anishchenko I, Coventry B, Cao L, Dauparas J, Halabiya S, DeWitt M, Carter L, Houk KN, Baker D. Nature. 2023 Feb;614(7949):774-780. doi: 10.1038/s41586-023-05696-3. Epub 2023 Feb 22. 10.1038/s41586-023-05696-3 PubMed 36813896
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