FCM101
(Plasmid #194298)

• Purpose: E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
• Depositing Lab: Shohei Koide
• Publication: Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi: 10.1038/s41467-022-33490-8.

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5234
• Total vector size (bp): 5609
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FCM101
• Species: Synthetic
• Insert Size (bp): 375
• Promoter: T7
• Tag / Fusion Protein:
  • His tag, FLAG tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7promoter
• 3′ sequencing primer: T7terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry