Hyper7 in pT3TS-Dest
(Plasmid
#194296)
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PurposeIn vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT3TS-Dest_R1-R3 (Addgene 140878)
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Vector typeIn vitro transcription of mRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHyper7
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hyper7 in pT3TS-Dest was a gift from Rob Parton (Addgene plasmid # 194296 ; http://n2t.net/addgene:194296 ; RRID:Addgene_194296) -
For your References section:
Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2. Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG. Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28. 10.1016/j.devcel.2023.02.004 PubMed 36858041