mKate2-DrCavin1a in pT3TS-Dest
(Plasmid
#194294)
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PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT3TS-Dest_R1-R3 (Addgene 140878)
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Vector typeIn vitro transcription of mRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecavin1a
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SpeciesD. rerio (zebrafish)
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Entrez Genecavin1a (a.k.a. ptrfa, si:ch211-209d14.2)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.06.09.447684v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKate2-DrCavin1a in pT3TS-Dest was a gift from Rob Parton (Addgene plasmid # 194294 ; http://n2t.net/addgene:194294 ; RRID:Addgene_194294) -
For your References section:
Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2. Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan MT, McMahon KA, Parton RG. Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28. 10.1016/j.devcel.2023.02.004 PubMed 36858041