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Addgene

U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
(Plasmid #194283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW; Addgene 71237
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 14927
  • Modifications to backbone
    Added Slc1a3 targeted sgRNA downstream of U6 promoter; replaced hUbC promoter with EF1a
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-KRAB-GFP
  • Alt name
    CRISPRi
  • gRNA/shRNA sequence
    non target sgRNA
  • Species
    M. musculus (mouse)
  • Promoter EF1a
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pacl (not destroyed)
  • 3′ cloning site Acc65I (not destroyed)
  • 5′ sequencing primer EF1a-F; U6-F
  • 3′ sequencing primer GFP-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.10.31.514562 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP was a gift from Elizabeth Kirby (Addgene plasmid # 194283 ; http://n2t.net/addgene:194283 ; RRID:Addgene_194283)

  • For your References section:

    Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal. Rieskamp JD, Rosado-Burgos I, Christofi JE, Ansar E, Einstein D, Walters AE, Valentini V, Bruno JP, Kirby ED. iScience. 2023 Jun 8;26(7):107068. doi: 10.1016/j.isci.2023.107068. eCollection 2023 Jul 21. 10.1016/j.isci.2023.107068 PubMed 37534178
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