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TDP-43 _CTD_5M→L_IDR
(Plasmid #194265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PJ411
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4452
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TDP-43
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Mutation
    M307L, M311L, M359L, M405L, M414L mutations in TDP-43 CTD
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Promoter T7
  • Tag / Fusion Protein
    • 6XHistag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDP-43 _CTD_5M→L_IDR was a gift from Nicolas Fawzi (Addgene plasmid # 194265 ; http://n2t.net/addgene:194265 ; RRID:Addgene_194265)

  • For your References section:

    A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain. Mohanty P, Shenoy J, Rizuan A, Mercado-Ortiz JF, Fawzi NL, Mittal J. Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2305625120. doi: 10.1073/pnas.2305625120. Epub 2023 Aug 14. 10.1073/pnas.2305625120 PubMed 37579155
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