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BacPAK HF-Uch37C88A
(Plasmid #19426)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BacPAK8
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Uch37
  • Alt name
    Uchl5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    C88A
  • GenBank ID
    Uchl5
  • Entrez Gene
    UCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer Bac1
  • 3′ sequencing primer Bac2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use translation from NM_015984, which corresponds to NP_057068, to determine protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BacPAK HF-Uch37C88A was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 19426 ; http://n2t.net/addgene:19426 ; RRID:Addgene_19426)

  • For your References section:

    Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Yao T, Song L, Xu W, DeMartino GN, Florens L, Swanson SK, Washburn MP, Conaway RC, Conaway JW, Cohen RE. Nat Cell Biol. 2006 Sep . 8(9):994-1002. 10.1038/ncb1460 PubMed 16906146
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