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Addgene

SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)
(Plasmid #194233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTK0_027 Entry Vector
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258
  • Species
    Synthetic
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077) was a gift from Hana El-Samad (Addgene plasmid # 194233 ; http://n2t.net/addgene:194233 ; RRID:Addgene_194233)

  • For your References section:

    Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein. Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H. bioRxiv 2021.04.20.440678 10.1101/2021.04.20.440678
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