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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)
(Plasmid #194230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAN2414 Lentiviral Destination
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PGK-LCB3-SynNotch-T2A-mCherry
  • Species
    Synthetic
  • Mutation
    E45D mutation in LCB3
  • Promoter PGK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047) was a gift from Hana El-Samad (Addgene plasmid # 194230 ; http://n2t.net/addgene:194230 ; RRID:Addgene_194230)

  • For your References section:

    Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein. Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H. bioRxiv 2021.04.20.440678 10.1101/2021.04.20.440678
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