LCB3_v1.2 (MTK 3a) (pZYW045)
(Plasmid
#194228)
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PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTK0_027 Entry Vector
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLCB3_v1.2
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SpeciesSynthetic
- Promoter None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LCB3_v1.2 (MTK 3a) (pZYW045) was a gift from Hana El-Samad (Addgene plasmid # 194228 ; http://n2t.net/addgene:194228 ; RRID:Addgene_194228) -
For your References section:
Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein. Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H. bioRxiv 2021.04.20.440678 10.1101/2021.04.20.440678