PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)
(Plasmid
#194226)
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PurposeLentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAN2414 Lentiviral Destination
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePGK-ACE2-SynNotch-T2A-mCherry
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SpeciesSynthetic
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter PGK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.04.20.440678 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037) was a gift from Hana El-Samad (Addgene plasmid # 194226 ; http://n2t.net/addgene:194226 ; RRID:Addgene_194226) -
For your References section:
Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein. Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H. bioRxiv 2021.04.20.440678 10.1101/2021.04.20.440678