BacPAK GST-Uch37C
(Plasmid
#19422)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5400
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUch37 239-329
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Alt nameUchl5
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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GenBank IDUchl5
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Entrez GeneUCHL5 (a.k.a. CGI-70, INO80R, UCH-L5, UCH37)
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Bac1
- 3′ sequencing primer Bac2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BacPAK GST-Uch37C was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 19422 ; http://n2t.net/addgene:19422 ; RRID:Addgene_19422) -
For your References section:
Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Yao T, Song L, Xu W, DeMartino GN, Florens L, Swanson SK, Washburn MP, Conaway RC, Conaway JW, Cohen RE. Nat Cell Biol. 2006 Sep . 8(9):994-1002. 10.1038/ncb1460 PubMed 16906146