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pDuex2-iBB-GECO1
(Plasmid #194216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDuex2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6160
  • Total vector size (bp) 7637
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iBB-GECO1
  • Species
    Synthetic
  • Insert Size (bp)
    1479
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDuex2-iBB-GECO1 was a gift from Robert Campbell (Addgene plasmid # 194216 ; http://n2t.net/addgene:194216 ; RRID:Addgene_194216)

  • For your References section:

    A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. Hashizume R, Fujii H, Mehta S, Ota K, Qian Y, Zhu W, Drobizhev M, Nasu Y, Zhang J, Bito H, Campbell RE. Protein Sci. 2022 Oct;31(10):e4440. doi: 10.1002/pro.4440. 10.1002/pro.4440 PubMed 36173169
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