pcDNA3.1(+)-iBB-GECO1
(Plasmid
#194215)
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PurposeFar-red fluorescent Ca2+ ion indicator for mammalian cell expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5420
- Total vector size (bp) 6902
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiBB-GECO1
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SpeciesSynthetic
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Insert Size (bp)1482
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-iBB-GECO1 was a gift from Jin Zhang (Addgene plasmid # 194215 ; http://n2t.net/addgene:194215 ; RRID:Addgene_194215) -
For your References section:
A genetically encoded far-red fluorescent calcium ion biosensor derived from a biliverdin-binding protein. Hashizume R, Fujii H, Mehta S, Ota K, Qian Y, Zhu W, Drobizhev M, Nasu Y, Zhang J, Bito H, Campbell RE. Protein Sci. 2022 Oct;31(10):e4440. doi: 10.1002/pro.4440. 10.1002/pro.4440 PubMed 36173169
