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Addgene

pCALNL-Rheb(S16H)
(Plasmid #194204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCALNL-GFP
  • Backbone manufacturer
    Addgene; catalog no. 13770
  • Backbone size w/o insert (bp) 4698
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ras Homolog Enriched Protein in Brain (S16H)
  • Alt name
    Rheb(S16H)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2092
  • Mutation
    S16H
  • Entrez Gene
    RHEB (a.k.a. RHEB2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCALNL-Rheb(S16H) was a gift from Angelique Bordey (Addgene plasmid # 194204 ; http://n2t.net/addgene:194204 ; RRID:Addgene_194204)

  • For your References section:

    Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335. Epub 2016 Sep 19. 10.1073/pnas.1605740113 PubMed 27647922
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