GST-14-3-3wt (pEBG)
(Plasmid #1942)

• Depositing Lab: Joseph Avruch
• Publication: Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Backbone

• Vector backbone: pEBG
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 14-3-3 zeta
• Alt name: 14.3.3
• Alt name: Ywhaz
• Species: M. musculus (mouse)
• Entrez Gene: Ywhaz (a.k.a. 1110013I11Rik, 14-3-3zeta)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry