pGL3-pSMANTIS_1kb_1 KLF2mut 449,548
(Plasmid
#194199)
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PurposeIncludes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSMANTIS promoter (1kb)
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Alt nameMANTIS promoter (1kb)
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Alt nameAK125871 promoter (1kb)
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SpeciesH. sapiens (human)
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Mutation2 mutated KLF binding sites (position 449,548)
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Entrez GeneSMANTIS (a.k.a. ANXA4-AS, n342419)
- Promoter SMANTIS promoter (1kb), mutated
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-pSMANTIS_1kb_1 KLF2mut 449,548 was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194199 ; http://n2t.net/addgene:194199 ; RRID:Addgene_194199)