pGL3-pSMANTIS_1kb_KLF2mut 732
(Plasmid
#194195)
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PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSMANTIS promoter (1kb)
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Alt nameMANTIS promoter (1kb)
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Alt nameAK125871 promoter (1kb)
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SpeciesH. sapiens (human)
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Mutationmutated KLF binding sites (position 732)
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Entrez GeneSMANTIS (a.k.a. ANXA4-AS, n342419)
- Promoter SMANTIS promoter (1kb), mutated
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-pSMANTIS_1kb_KLF2mut 732 was a gift from Ralf Brandes & Matthias Leisegang (Addgene plasmid # 194195 ; http://n2t.net/addgene:194195 ; RRID:Addgene_194195) -
For your References section:
Pleiotropic effects of laminar flow and statins depend on the Kruppel-like factor-induced lncRNA MANTIS. Leisegang MS, Bibli SI, Gunther S, Pfluger-Muller B, Oo JA, Hoper C, Seredinski S, Yekelchyk M, Schmitz-Rixen T, Schurmann C, Hu J, Looso M, Sigala F, Boon RA, Fleming I, Brandes RP. Eur Heart J. 2019 Aug 7;40(30):2523-2533. doi: 10.1093/eurheartj/ehz393. 10.1093/eurheartj/ehz393 PubMed 31222221